期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 15, 期 2, 页码 2773-2793出版社
MDPI
DOI: 10.3390/ijms15022773
关键词
gene knockout; allelic exchange; gene knock-in; genome editing; RNA-guided nucleases; mobile group II intron; antisense RNA; gene knockdown; gene silencing
资金
- Japan Society for the Promotion of Science (JSPS) [23780096, 23380197, 24119515, 24651231]
- Grants-in-Aid for Scientific Research [23380197, 23780096, 24119515, 24651231] Funding Source: KAKEN
Genome editing is an important technology for bacterial cellular engineering, which is commonly conducted by homologous recombination-based procedures, including gene knockout (disruption), knock-in (insertion), and allelic exchange. In addition, some new recombination-independent approaches have emerged that utilize catalytic RNAs, artificial nucleases, nucleic acid analogs, and peptide nucleic acids. Apart from these methods, which directly modify the genomic structure, an alternative approach is to conditionally modify the gene expression profile at the posttranscriptional level without altering the genomes. This is performed by expressing antisense RNAs to knock down (silence) target mRNAs in vivo. This review describes the features and recent advances on methods used in genomic engineering and silencing technologies that are advantageously used for bacterial cellular engineering.
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