期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 16, 期 1, 页码 547-563出版社
MDPI
DOI: 10.3390/ijms16010547
关键词
Ocimum basilicum; essential oil; beta-cyclodextrin; pain; fos protein; fibromyalgia
资金
- CNPq/Brazil
- CAPES/Brazil
- FAPITEC/Brazil
O. basilicum leaves produce essential oils (LEO) rich in monoterpenes. The short half-life and water insolubility are limitations for LEO medical uses. beta-Cyclodextrin (beta-CD) has been employed to improve the pharmacological properties of LEO. We assessed the antihyperalgesic profile of LEO, isolated or complexed in beta-CD (LEO/beta-CD), on an animal model for fibromyalgia. Behavioral tests: mice were treated every day with either LEO/beta-CD (25, 50 or 100 mg/kg, p.o.), LEO (25 mg/kg, p.o.), tramadol (TRM 4 mg/kg, i.p.) or vehicle (saline), and 60 min after treatment behavioral parameters were assessed. Therefore, mice were evaluated for mechanical hyperalgesia (von Frey), motor coordination (Rota-rod) and muscle strength (Grip Strength Metter) in a mice fibromyalgia model. After 27 days, we evaluated the central nervous system (CNS) pathways involved in the effect induced by experimental drugs through immunofluorescence protocol to Fos protein. The differential scanning analysis (DSC), thermogravimetry/derivate thermogravimetry (TG/DTG) and infrared absorption spectroscopy (FTIR) curves indicated that the products prepared were able to incorporate the LEO efficiently. Oral treatment with LEO or LEO-beta CD, at all doses tested, produced a significant reduction of mechanical hyperalgesia and we were able to significantly increase Fos protein expression. Together, our results provide evidence that LEO, isolated or complexed with beta-CD, produces analgesic effects on chronic non-inflammatory pain as fibromyalgia.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据