期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 15, 期 3, 页码 5063-5078出版社
MDPI AG
DOI: 10.3390/ijms15035063
关键词
abiotic stress tolerance; heat shock protein; transformation
资金
- National Natural Science Foundation of China [31272202]
- Program for Hi-Tech Research, Jiangsu, China [BE2012350, BE2011325]
- Program for New Century Excellent Talents in University of the Chinese Ministry of Education [NCET-10-0492]
Heat shock proteins are associated with protection against various abiotic stresses. Here, the isolation of a chrysanthemum cDNA belonging to the HSP70 family is reported. The cDNA, designated CgHSP70, encodes a 647-residue polypeptide, of estimated molecular mass 70.90 kDa and pI 5.12. A sub-cellular localization assay indicated that the cDNA product is deposited in the cytoplasm and nucleus. The performance of Arabidopsis thaliana plants constitutively expressing CgHSP70 demonstrated that the gene enhances tolerance to heat, drought and salinity. When CgHSP70 was stably over-expressed in chrysanthemum, the plants showed an increased peroxidase (POD) activity, higher proline content and inhibited malondialdehyde (MDA) content. After heat stress, drought or salinity the transgenic plants were better able to recover, demonstrating CgHSP70 positive effect.
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