期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 15, 期 7, 页码 11742-11759出版社
MDPI AG
DOI: 10.3390/ijms150711742
关键词
silica; titanium oxide; nano; toxicity; neural stem cell; neural progenitor cell; differentiation
资金
- Health and Labor Sciences Research Grants from the Ministry of Health, Labor and Welfare of Japan [H22-chemical-young-009]
- Japan Science Society
Several in vivo studies suggest that nanoparticles (smaller than 100 nm) have the ability to reach the brain tissue. Moreover, some nanoparticles can penetrate into the brains of murine fetuses through the placenta by intravenous administration to pregnant mice. However, it is not clear whether the penetrated nanoparticles affect neurogenesis or brain function. To evaluate its effects on neural stem cells, we assayed a human neural stem cell (hNSCs) line exposed in vitro to three types of silica particles (30 nm, 70 nm, and <44 mu m) and two types of titanium oxide particles (80 nm and < 44 mu m). Our results show that hNSCs aggregated and exhibited abnormal morphology when exposed to the particles at concentrations >= 0.1 mg/mL for 7 days. Moreover, all the particles affected the gene expression of Nestin (stem cell marker) and neurofilament heavy polypeptide (NF-H, neuron marker) at 0.1 mg/mL. In contrast, only 30-nm silica particles at 1.0 mg/mL significantly reduced mitochondrial activity. Notably, 30-nm silica particles exhibited acute membrane permeability at concentrations >= 62.5 mu g/mL in 24 h. Although these concentrations are higher than the expected concentrations of nanoparticles in the brain from in vivo experiments in a short period, these thresholds may indicate the potential toxicity of accumulated particles for long-term usage or continuous exposure.
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