期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 14, 期 4, 页码 8603-8610出版社
MDPI AG
DOI: 10.3390/ijms14048603
关键词
Salmonella; fluorescent immunoassay; magnetic separation; quantum dots
资金
- National Natural Science Foundation of China [21071066, 91027038, 21101079, 21175034]
- MOST [2012BAC01B07, 2012BAD29B05, 2012AA06A303, 2012BAD29B04, 2011BAK10B07, 2011BAK10B05, 2011BAK10B01, 2010AA06Z302, 2010DFB3047, 2011ZX08012-001, 2012BAK17B10, 2012BAK08B01, 2012YQ090194]
- Natural Science Foundation of Jiangsu Province
- MOF
- MOE [NCET-12-0879, BE2011626, 201210036, 201310135, 311002]
- Foundation for the Author of National Excellent Doctoral Dissertation of China [201187]
- State Key Laboratory of Food Science and Technology, Jiangnan University [SKLF-ZZA-201202]
Simple immuno-magnetic separation tandem fluorescent probes based on quantum dots-antibody (QDs-Ab) were developed to detect Salmonella with sensitivity of 500 cfu mL(-1). With two monoclonal antibodies, which recognize different antigenic determinant on the surface of Salmonella, we prepared antibody-coated magnetic nanoparticles (MNPs) and conjugates of QDs-Ab. The immune-magnetic beads were verified with high enrichment efficiency for Salmonella (90%). A sandwich structure formed if the Salmonella solution was mixed together with immune-beads and QDs-Ab, and the fluorescent single from QDs was related to the amount of Salmonella. A linear response between fluorescence intensity and various concentrations of Salmonella (2.5 x 10(3) to 1.95 x 10(8) cfu mL(-1)) were observed with this proposed method. The total assay time for Salmonella was 30 min, and no cross-reaction to other microbial strains, such as Staphylococcus aureus, Escherichia coli (E. coli) and Escherichia coli O157:H7 (E. coli O157:H7), were found using this detection system. All our results showed that the simple homogeneous immunoassay could be applied in Salmonella screening without time-consuming extra-enrichment of bacteria.
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