期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 14, 期 12, 页码 24242-24254出版社
MDPI
DOI: 10.3390/ijms141224242
关键词
dynein; -synuclein; Parkinson's disease; autophagy; autolysosome
资金
- National Natural Science Foundation of China [81171213, 81171212]
- Natural Science Foundation of Jiangsu Province of China [2010228]
- Suzhou Science and Technology Development Program [SZS201205, SYS201126]
- National Basic Science Key Project (973 project) [2011CB510003]
Growing evidence suggests that dynein dysfunction may be implicated in the pathogenesis of neurodegeneration. It plays a central role in aggresome formation, the delivery of autophagosome to lysosome for fusion and degradation, which is a pro-survival mechanism essential for the bulk degradation of misfolded proteins and damaged organells. Previous studies reported that dynein dysfuntion was associated with aberrant aggregation of -synuclein, which is a major component of inclusion bodies in Parkinson's disease (PD). However, it remains unclear what roles dynein plays in -synuclein degradation. Our study demonstrated a decrease of dynein expression in neurotoxin-induced PD models in vitro and in vivo, accompanied by an increase of -synuclein protein level. Dynein down-regulation induced by siRNA resulted in a prolonged half-life of -synuclein and its over-accumulation in A53T overexpressing PC12 cells. Dynein knockdown also prompted the increase of microtubule-associated protein 1 light chain 3 (LC3-II) and sequestosome 1 (SQSTM1, p62) expression, and the accumulation of autophagic vacuoles. Moreover, dynein suppression impaired the autophagosome fusion with lysosome. In summary, our findings indicate that dynein is critical for the clearance of aberrant -synuclein via autophagosome-lysosome pathway.
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