期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 14, 期 10, 页码 20399-20413出版社
MDPI
DOI: 10.3390/ijms141020399
关键词
Calanthe tsoongiana; genetic diversity; ISSR; conservation; Orchidaceae
资金
- Key Science and Technology Specific Projects of Zhejiang Province [2012C12909-10]
- Science and Technology Projects of Zhejiang Forestry Department [2012Y02-3]
Calanthe tsoongiana is a rare terrestrial orchid endemic to China, and this species has experienced severe habitat loss and fragmentation. Inter-simple sequence repeat (ISSR) markers were employed to assess the genetic diversity and differentiation of six populations of C. tsoongiana. Based on 124 discernible fragments yielded by eleven selected primers, high genetic diversity was revealed at the species level; however, genetic diversity at the population level was relatively low. High-level genetic differentiation among populations was detected based on analysis of molecular variance (AMOVA), indicating potential limited gene flow. No significant relationship was observed between genetic and geographic distances among the sampled populations. These results suggested that restricted gene flow might be due to habitat fragmentation and reduced population size as a result of human activities. Based on the findings, several conservation strategies were proposed for the preservation of this threatened species.
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