The Effect of the Aerial Part of Lindera akoensis on Lipopolysaccharides (LPS)-Induced Nitric Oxide Production in RAW264.7 Cells

Four new secondary metabolites, 3 alpha-((E)-Dodec-1-enyl)-4 beta-hydroxy-5 beta-methyldihydrofuran-2-one (1), linderinol (6), 4'-O-methylkaempferol 3-O-alpha-L-(4 ''-E-p-coumaroyl) rhamnoside (11) and kaempferol 3-O-alpha-L-(4 ''-Z-p-coumaroyl) rhamnoside (12) with eleven known compounds-3-epilistenolide D-1 (2), 3-epilistenolide D-2 (3), (3Z, 4 alpha, 5 beta)-3-(dodec-11-ynylidene)-4-hydroxy-5-methylbutanolide (4), (3E, 4 beta, 5 beta)-3-(dodec-11-ynylidene)-4-hydroxy-5-methylbutanolide (5), matairesinol (7), syringaresinol (8), (+)-pinoresinol (9), salicifoliol (10), 4 ''-p-coumaroylafzelin (13), catechin (14) and epicatechin (15)-were first isolated from the aerial part of Lindera akoensis. Their structures were determined by detailed analysis of 1D-and 2D-NMR spectroscopic data. All of the compounds isolated from Lindera akoensis showed that in vitro anti-inflammatory activity decreases the LPS-stimulated production of nitric oxide (NO) in RAW 264.7 cell, with IC50 values of 4.1-413.8 mu M.