期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 14, 期 3, 页码 5899-5919出版社
MDPI
DOI: 10.3390/ijms14035899
关键词
FK506 binding protein; FKBP16-3; thylakoid lumen; environmental stress tolerance
资金
- Next Generation of Bio Green 21 Project
- National Center for GM Crops from RDA [PJ009043]
- National Research Foundation of Korea (NRF)
- Korea government (MEST) [2012R1A1A2044517]
- Cabbage Genomics assisted breeding supporting center (CGC) research programs
- Ministry for Food, Agriculture, Forestry and Fisheries of the Korean Government
- KRIBB Initiative Program
- National Research Foundation of Korea [2012R1A1A2044517] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The putative thylakoid lumen immunophilin, FKBP16-3, has not yet been characterized, although this protein is known to be regulated by thioredoxin and possesses a well-conserved CxxxC motif in photosynthetic organisms. Here, we characterized rice OsFKBP16-3 and examined the role of this gene in the regulation of abiotic stress in plants. FKBP16-3s are well conserved in eukaryotic photosynthetic organisms, including the presence of a unique disulfide-forming CxxxC motif in their N-terminal regions. OsFKBP16-3 was mainly expressed in rice leaf tissues and was upregulated by various abiotic stresses, including salt, drought, high light, hydrogen peroxide, heat and methyl viologen. The chloroplast localization of OsFKBP16-3-GFP was confirmed through the transient expression of OsFKBP16-3 in Nicotiana benthamiana leaves. Transgenic Arabidopsis and transgenic rice plants that constitutively expressed OsFKBP16-3 exhibited increased tolerance to salinity, drought and oxidative stresses, but showed no change in growth or phenotype, compared with vector control plants, when grown under non-stressed conditions. This is the first report to demonstrate the potential role of FKBP16-3 in the environmental stress response, which may be regulated by a redox relay process in the thylakoid lumen, suggesting that artificial regulation of FKBP16-3 expression is a candidate for stress-tolerant crop breeding.
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