期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 14, 期 6, 页码 10809-10818出版社
MDPI
DOI: 10.3390/ijms140610809
关键词
T-2 toxin; chicken CYP1A5; metabolism
资金
- National Basic Research Program of China (973 Program) [2009CB118802]
- National Natural Science Foundation of China [31172087]
The transmission of T-2 toxin and its metabolites into the edible tissues of poultry has potential effects on human health. We report that T-2 toxin significantly induces CYP1A4 and CYP1A5 expression in chicken embryonic hepatocyte cells. The enzyme activity assays of CYP1A4 and CYP1A5 heterologously expressed in HeLa cells indicate that only CYP1A5 metabolizes T-2 to 3'OH-T-2 by the 3'-hydroxylation of isovaleryl groups. In vitro enzyme assays of recombinant CYP1A5 expressed in DH5 alpha further confirm that CYP1A5 can convert T-2 into TC-1 (3'OH-T-2). Therefore, CYP1A5 is critical for the metabolism of trichothecene mycotoxin in chickens.
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