期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 13, 期 11, 页码 14401-14420出版社
MDPI
DOI: 10.3390/ijms131114401
关键词
Caco-2; secretome; differentiation; DIGE; colorectal cancer; biomarkers
资金
- Xunta de Galicia
- FEDER [10 PXIB 310 215 PR, CN2011/024]
- Maria Barbeito program (Xunta de Galicia)
- Angeles Alvarino program (Xunta de Galicia)
Colorectal cancer is still a major health burden worldwide, and its diagnosis has not improved in recent years due to a lack of appropriate diagnostic serum markers. Aiming to find new diagnostic proteins, we applied the proteomic DIGE technology to analyze changes in the secretome before/after differentiation of the colon adenocarcinoma Caco-2 cell line, an accepted in vitro model to study colorectal tumorigenesis. When the secretomes from undifferentiated (tumor-like) and differentiated cells (resembling healthy enterocytes) were compared, we found 96 spots differentially expressed. After MS/MS analysis, 22 spots corresponding to 15 different proteins were identified. Principal component analysis demonstrated these 22 spots could serve as a discriminatory panel between the tumor-like and normal-like cells. Among the identified proteins, the translationally-controlled tumor protein (TCTP), the transforming growth factor-beta-induced protein ig-h3 (TGF beta Ip), and the Niemann-Pick disease type C2 protein (NPC2) are interesting candidates for future studies focused on their utility as serum biomarkers of colorectal cancer.
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