期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 13, 期 6, 页码 7762-7775出版社
MDPI
DOI: 10.3390/ijms13067762
关键词
B. mori fibroin; silk gland; porous scaffolds; compressive strength; cell adhesion and growth activity; ALP activity
资金
- National Natural Science Foundation of China [20804037, 21172194]
- Research Fund for the Doctoral Program of Higher Education of China [200803351017]
- Fundament Research Funds for the Center Universities [2009QNA6037]
In order to use a simple and ecofriendly method to prepare porous silk scaffolds, aqueous silk fibroin solution (ASF) was extracted from silk gland of 7-day-old fifth instar larvae of Bombyx mori (B. mori). SDS-page analysis indicated that the obtained fibroin had a molecular weight higher than 200 kDa. The fabrication of porous scaffolds from ASF was achieved by using the freeze-drying method. The pore of porous scaffolds is homogenous and tends to become smaller with an increase in the concentration of ASF. Conversely, the porosity is decreased. The porous scaffolds show impressive compressive strength which can be as high as 6.9 +/- 0.4 MPa. Furthermore, ASF has high cell adhesion and growth activity. It also exhibits high ALP activity. This implies that porous scaffolds prepared from ASF have biocompatibility. Therefore, the porous scaffolds prepared in this study have potential application in tissue engineering due to the impressive compressive strength and biocompatibility.
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