期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 9, 期 4, 页码 571-577出版社
MDPI AG
DOI: 10.3390/ijms9040571
关键词
glycyrrhizic acid; glabridin; licorice; extraction; high-performance liquid chromatography
资金
- National Research Foundation of Korea [R11-2000-068-04003-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The extraction and separation conditions of glycyrrhizic acid and glabridin from licorice were investigated. By changing the different extraction solvents, procedures, times and temperature, the optimum extraction condition was established: the used of ethanol/water (30: 70, v/v) as an extraction solvent, and 60 min dipping time under 50 C. The extracts of licorice were separated and determined by reversed-phase high performance liquid chromatography with a methanol/water (70: 30, v/v, containing 1% acetic acid) as the mobile phase. Under the optimum extraction condition, 2.39 mg/g of glycyrrhizic acid and 0.92 mg/g of glabridin were extracted from Chinese licorice and the recoveries were 89.7% and 72.5% respectively.
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