期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 30, 期 5, 页码 1053-1060出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2012.1088
关键词
human immunodeficiency virus type 1 integrase; SUMO; Ubc9; integration; lentivirus
资金
- National Basic Research Program of China
- project 973 [2010CB529903]
- project 863 [2007aa021002/2009zx09503-020]
- Nature Science Fund [30971617]
Human immunodeficiency virus type I (HIV-I) integrase mediates the integration of reverse-transcribed viral cDNA into the genome of the host for the stable maintenance of the viral genome and the persistence of HIV-1 infection. In this study, tie relationships between HIV-I integrase (HIV-I IN) and three SUMO conjugation pathway proteins, as well as the effects of these associations, were investigated. The overexpression of SUMO1/SUMO2 and Ubc9 changed the intracellular localization of HIV-I IN from a diffuse distribution to a puncta:e localization. SUMO1, SUMO2 and Ubc9 were shown to interact with HIV-I IN. The SUMOylation of HIV-1 IN was verified. In addition, SUMO1, SUMO2 and Ubc9 were shown to influence the integration of both lentivirus and HIV-I. The overexpression of Ubc9 inhibited viral genome integration, and the upregulation of SUMO1 or SUMO2 enhanced the inhibitory effect of Ubc9. Knockdown of the endogenous levels of SUMO1, SUMO2 and Ubc9 increased the level of viral integration, while reverse transcription and the nuclear import of preintegration complex (PIC) were not affected. Our findings suggest that SUMO conjugation pathway proteins may act as cellular restriction factors and be detrimental to HIV-1 infection. These findings merit further investigation because of their potentially significant implications for the cellular antiviral response to HIV-1 infection.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据