期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 30, 期 5, 页码 1145-1151出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2012.1116
关键词
beta B2-crystallin; proliferation; apoptosis; Bcl-2; calcium; Ca2+-calmodulin dependent protein kinase IV
资金
- National Natural Science Foundation of China [81170834]
- Shanghai Municipal Science and Technology Commission of China [11ZR1447500]
beta B2-crystallin (Crybb2), a member of the beta gamma-crystallin superfamily, in conjunction with a-crystallin, constitute the major proteins of the mammalian eye lens. Crybb2 is also expressed outside the lens, and certain related functions in these tissues have been reported. In the present study, in order to define the physiological role of Crybb2, we generated mice with a targeted deletion of the Crybb2 gene. Surprisingly, fertility was markedly reduced in male homozygous knockout mice compared to wild-type (WT) mice. Further experiments were performed to explore the underlying mechanism of subfertility in male Crybb2(-/-) mice. Our results showed that Crybb2 was mainly expressed in the spermatogonia from the testes of mice with the WT C57BL/C genetic background. The testes of 4-week-old Crybb2(-/-) mice were significantly hyperplastic, and no significant difference was found within 3 weeks postpartum. Additionally, there was a marked increase in the proliferation and apoptosis of germ cells, and the biological defects of these cells correlated with the decreased Bcl-2 levels, which correlated with the reduction of Ca2+-calmodulin-dependent protein kinase IV (CaMKIV) in the testis. These results suggest that the reduced fertility of Crybb2(-/-) male mice may result from the disordered proliferation and apoptosis of germ cells in the testis, possibly due to reduced CaMKIV from the loss of Crybb2.
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