期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 27, 期 3, 页码 393-399出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2011.598
关键词
angiotensin II; pre-eclampsia; extracellular signal-related kinase 1/2; miR-155; angiotensin II type 1 receptor
资金
- Shanghai Municipal Health Bureau [2008Y002]
- Medicine-Engineering Unite Fund for Shanghai Jiaotong University [YG2009MS47]
- Natural Science Foundation of School of Medicine of Shanghai Jiaotong University
- Shanghai Committee Medical Science Foundation of China [10411967100]
- National Key Technology RD Program [2006BAI05A05]
Angiotensin II is critical in pre-eclampsia pathogenesis. In addition, microRNA-155 regulates angiotensin II type I receptor expression. We have explored the function of microRNA-155 in pre-eclamptic pregnant women. Human umbilical vein endothelial cells were isolated and cultured from healthy puerperant women and pre-eclampsia patients. The cells were transfected with a mature microRNA-155 plasmid. The effect of microRNA-155 was assessed by Northern blotting, in situ hybridization, quantitative real-time PCR, immunofluorescent staining and Western blotting. In addition, activation of extracellular signal-regulated kinase 1/2 was assessed by co-immunoprecipitation and Western blotting. Severely pre-eclamptic pregnant women expressed less mature miR-155 compared to healthy pregnant women. In addition, angiotensin H type 1 receptor expression decreased substantially in healthy cells and miR-155-transfected cells compared to miR-155-mutant-transfected cells and cells from pre-eclamptic patients. Mature miR-155 reduced angiotensin II-induced extracellular signal-regulated kinase 1/2 activation. In conclusion, endogenous mature miR-155 expression may be an important contributor to the pathogenesis of severe pre-eclampsia.
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