期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 27, 期 3, 页码 423-428出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2011.595
关键词
heat shock protein 27; transforming growth factor-beta; vascular endothelial growth factor; osteoblast
资金
- Ministry of Education, Science, Sports and Culture of Japan [16590873, 16591482]
- Ministry of Health, Labour and Welfare of Japan [21A-4, 21A-22]
- Grants-in-Aid for Scientific Research [16590873] Funding Source: KAKEN
We have previously reported that transforming growth factor-beta (TGF-beta) stimulates heat shock protein 27 (HSP27) induction via p44/p42 mitogen-activated protein (MAP) kinase, p38 MAP kinase and stress-activated protein kinase/c-Jun N-terminal kinase in osteoblast-like MC3T3-E1 cells, and that the release of vascular endothelial growth factor (VEGF) is induced by TGF-beta in these cells. In the present study, we investigated the effect of HSP27 knockdown on the TGF-beta-stimulated VEGF release in these cells. Gene silencing using short interfering RNA against HSP27 (HSP27-siRNA) significantly suppressed the TGF-beta-induced VEGF release. Immunofluorescence microscopy also revealed that HSP27-siRNA suppressed the TGF-beta-stimulated VEGF induction as well as the reduction of HSP27 induction in these cells. However, the mRNA expression of VEGF stimulated by TGF-beta was not reduced even in cells transfected with HSP27-siRNA. These results strongly suggest that HSP27 induction is critical for TGF-beta-induced VEGF release in osteoblasts.
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