期刊
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
卷 8, 期 1, 页码 56-67出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijms.8.56
关键词
Helicobacter pylori; Reactive Oxygen Species; Mitochondrial DNA; Mutation
资金
- Shanghai Science and Technology Commission [08411968100]
To investigate the role of ROS in the helicobacter pylori (Hp) induced mtDNA mutations, AGS cells were treated by extracts of Hp11638 or Hp11638M. The ROS levels, cytochrome C reductions, and intracellular ATP levels were measured. The coding region and the D-Loop region were amplified and sequenced. Results showed the ROS levels, cytochrome C reduction and mtDNA mutations were markedly increased and cell viability decreased after treatment with both Hp extracts, and 616 mutations were detected in D-Loop region and 3 heteroplasmic point mutations in the Cytb gene. No mutations were found in the coding region. The mutation rates of mtDNA D-Loop region were positively correlated with the ROS levels and negatively to the ATP levels.
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