期刊
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
卷 345, 期 -, 页码 45-53出版社
ELSEVIER
DOI: 10.1016/j.ijms.2012.09.012
关键词
Oligonucleotide; Bioanalysis; Quantification; Accurate-mass LC-MS; LC-MS/MS
Quantification of oligonucleotide strands from rat plasma has been assessed on two tandem quadrupole systems and a TOF-MS platform following a novel ion-exchange solid-phase extraction and ion-pair chromatographic separation. Limits of quantification are comparable across all three systems with excellent signal-to-noise and accuracy and precision data realised on the TOF-MS to 10 ng/mL. Despite the advances in tandem quadrupole performance in recent years this has not translated into a marked improvement in the detection limits on this instrument geometry. This may prove to be a feature of the tandem MS process for oligonucleotides and could limit the ability to realise lower detection limits on these types of instruments. (C) 2012 Elsevier B.V. All rights reserved.
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