期刊
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
卷 312, 期 -, 页码 201-207出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijms.2011.05.013
关键词
Clinical analysis; Ambient ionization; Electrophoresis; Paper spray; In situ analysis
资金
- National Science Foundation [CHE 0847205, CHE 0848650, DBI 0852740]
- National Institutes of Health [1R21RR031246-01, 1R21EB009459-01]
- Alfred Mann Institute at Purdue
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0848650, 847205] Funding Source: National Science Foundation
- Div Of Biological Infrastructure
- Direct For Biological Sciences [0852740] Funding Source: National Science Foundation
Paper spray ionization has been developed as a direct, fast and low-cost sampling and ionization method for qualitative and quantitative mass spectrometric (MS) analysis of complex mixtures. Analyte ions are generated by applying a high voltage and a small volume (similar to 10 mu L) of spray solvent onto a porous substrate. The sample can be preloaded onto the paper or mixed into the spray solution. The geometry of the paper and the method of supplying the necessary internal standard are important factors that affect the ionization efficiency and subsequently the sensitivity and quantitation accuracy of the analytical data. As the cut angle of the paper tip is changed, the spray plume, the total spray current and the electric field intensity at the tip all vary correspondingly, with resulting differences in signal intensity. Sample load is another important factor for obtaining a stable MS signal and accurate quantitative results. The optimal sample load was found to be dependent on the paper size. The dissolution and spray process was also investigated and analyte transfer on paper was shown to be largely associated with bulk solution flow toward the spray tip. The information gathered from these systematic studies provides guidance for the design and optimization of a disposable sample cartridge for paper spray MS, a device which potentially is suitable for fast clinical analysis, especially for point-of-care diagnostics. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据