期刊
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
卷 305, 期 2-3, 页码 103-108出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijms.2010.09.008
关键词
Diacylglycerol; Normal phase; Electrospray; Diflurophenyl urethane derivative; Neutral loss; Lipid; Monoacylglycerol; Diacylglycerol
资金
- National Institutes of Health [U54GM069338]
The quantitative determination of 48 molecular species of regioisomeric diacylglycerols has been made in a single analysis of an extract of bone marrow derived macrophages. The analytical procedure involves solvent extraction of neutral lipids, including diacylglycerols, derivatization of free hydroxyl moieties as 2,4-difluorophenyl urethane, and analysis by normal phase liquid chromatography tandem mass spectrometry. The derivatization step not only prevents fatty acyl group migration, thus allowing determination of both 1,2- and 1,3-diacylglycerols, but also yields species that are sensitively and uniquely determined by constant neutral loss mass spectrometry. The method also detected monoacylglycerols, which were characterized by unique retention time and collisional spectra, and were present in mouse bone marrow derived macrophage extracts. (C) 2010 Elsevier B.V. All rights reserved.
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