期刊
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
卷 123, 期 1, 页码 71-74出版社
SPRINGER
DOI: 10.1007/s00414-008-0249-z
关键词
Body fluid; Cellular origin; mRNA markers; Blood; Saliva; RNA stability
资金
- Netherlands Forensic Institute
- Erasmus University Medical Center Rotterdam
In forensic science, the unequivocal identification of the cellular origin of crime scene samples used for DNA profiling can provide crucial information for crime scene reconstruction. We have previously shown that various mRNA markers from genes with expression patterns specific for blood and saliva can be established from whole-genome expression analysis of time-wise degraded samples and were stable enough to specifically identify blood and saliva stains up to 180 days of age. Here, we showed that nine blood-specific and five saliva-specific mRNA markers can be amplified successfully and reliably in much older blood (13-16 years) and saliva (2-6 years) stains, respectively, suggesting their suitability for tissue identification in forensic case work. Moreover, our findings imply that forensic RNA testing can be reliable and robust if degraded samples are considered in the marker ascertainment procedure, with promising expectations beyond tissue identification purposes.
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