期刊
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
卷 15, 期 6, 页码 E388-E394出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ijid.2011.02.003
关键词
Copy number variation; Human beta-defensin 3; Staphylococcus aureus; Pyrosequencing-based paralog ratio test
资金
- Det Sundhedsvidenskabelige Forskningsrad
- Idella Foundation
Objective: The DEFB103 gene encodes human beta-defensin 3, which has a high activity against Staphylococcus aureus. In the general population 20% are persistent nasal carriers of S. aureus, which is a problem for their general health. DEFB103 shows extensive variation in copy number. Copy number variations (CNVs) are believed to play a role in susceptibility to certain diseases. The possible associations between CNVs, mRNA, and nasal S. aureus carriage status were investigated. Methods: We used the pyrosequencing-based paralog ratio test to determine the DEFB103 copy number. Nasal swabs were collected for RNA and S. aureus determination. S. aureus genotypes were determined by spa typing, and real-time PCR was used to determine DEFB103 mRNA expression. Results: The DEFB103 CNV varied from 2 to 8 copies per diploid genome. No significant difference in copy number was observed among the groups. We found 74% of the volunteers to be non-carriers, 20% to be persistent carriers, and 6% to be intermittent carriers. The S. aureus isolates linked to more than 16 clonal lineages. mRNA expression varied extensively, but no significant differences were observed between the groups. We did not find a linear correlation between CNV and mRNA expression. Conclusions: The results indicate that DEFB103 CNV does not influence S. aureus carrier status. (C) 2011 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
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