期刊
INTERNATIONAL JOURNAL OF HYPERTHERMIA
卷 25, 期 5, 页码 355-363出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/02656730902964357
关键词
Thermal ablation; conductive interstitial thermal therapy (CITT); triphenyl tetrazolium chloride (TTC); autofluorescence; heat-fixed; VX2 carcinoma; surgical margins
资金
- National Cancer Institute NIH/NCI [CA108678-02, CA44114]
- NATIONAL CANCER INSTITUTE [R21CA108678, R01CA044114] Funding Source: NIH RePORTER
Purpose: A proof-of-concept study to evaluate a new autofluorescence method to differentiate necrotic thermally fixed cells from viable tissue following thermal ablation. Methods: A conductive interstitial thermal therapy (CITT) device was used to ablate swine mammary tissue and rabbit VX-2 carcinomas in vivo. The ablated regions and 10-mm margins were resected 24 h following treatment, embedded in HistOmer (R) and sectioned at 3mm. The fresh sections were evaluated for gross viability with triphenyl tetrazolium chloride, 1 h post-resection. Representative non-viable and viable areas were then processed and embedded into paraffin, and sectioned at 5 mm. Standard H&E staining and proliferating cell nuclear antigen (PCNA) immunohistochemistry were compared against autofluorescence intensity, at 488-nm wavelength, for cellular viability. Results: Heat-fixed cells in non-viable regions exhibit increased autofluorescence intensity compared to viable tissue (area under receiver operating characteristics (ROC) curve = 0.96; Mann-Whitney P < 0.0001). An autofluorescence intensity-based classification rule achieved 92% sensitivity with 100% specificity for distinguishing non-viable from viable samples. In contrast, PCNA staining did not reliably distinguish heat-fixed, dead cells from viable cells. Conclusions: Examination of H&E-stained sections using autofluorescence intensity-based classification is a reliable and readily available method to accurately identify heat-fixed cells in ablated surgical margins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据