期刊
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
卷 216, 期 5, 页码 541-552出版社
ELSEVIER GMBH
DOI: 10.1016/j.ijheh.2013.01.008
关键词
Micronucleus; Cytokinesis-block; Automation; Image cytometry; Lymphocytes
资金
- Grant Agency of the Czech Republic [P503/11/0084]
- European Union's Seventh Framework Programme (FP7) [241536]
- EU Integrated Project NewGeneris, 6th Framework Programme, Priority 5: Food Quality and Safety [FOOD-CT-2005-016320]
- AIRC (Associazione Italiana per la Ricerca sul Cancro)
- INAIL (Istituto Nazionale Assicurazione sul Lavoro)
- Italian Association for Research on Cancer (AIRC) [IG 5706 2008]
- Australia's National Health and Medical Research Council [APP 1037979]
- European Union Framework 7 program [289511]
- National Institute of Allergy and Infectious diseases (NIAID)
- Department of Defence, USA through Uniformed Service University of Health Sciences, Bethesda, USA
- Qatar Foundation
- MetaSystems
- IMSTAR
The use of micronucleus (MN) assays in in vitro genetic toxicology testing, radiation biodosimetry and population biomonitoring to study the genotoxic impacts of environment gene-interactions has steadily increased over the past two decades. As a consequence there has been a strong interest in developing automated systems to score micronuclei, a biomarker of chromosome breakage or loss, in mammalian and human cells. This paper summarises the outcomes of a workshop on this topic, organised by the HUMN project, at the 6th International Conference on Environmental Mutagenesis in Human Populations at Doha, Qatar, 2012. The aim of this paper is to summarise the outcomes of the workshop with respect to the set objectives which were: (i) Review current developments in automation of micronucleus assays by image cytometry; (ii) define the performance characteristics of automated MN scoring using image cytometry and methods of assessment for instrument validation and quality control and (iii) discuss the design of inter-laboratory comparisons and standardisation of micronucleus assays using automated image cytometry systems. It is evident that automated scoring of micronuclei by automated image cytometry using different commercially available platforms [e.g. Metafer (MetaSystems), Pathfinder (TM) (IMSTAR), iCyte (R) (Compucyte)], particularly for lymphocytes, is at a mature stage of development with good agreement between visual and automated scoring across systems (correlation factors ranging from 0.58 to 0.99). However, a standardised system of validation and calibration is required to enable more reliable comparison of data across laboratories and across platforms. This review identifies recent progress, important limitations and steps that need to be taken into account to enable the successful universal implementation of automated micronucleus assays by image cytometry. Crown Copyright (c) 2013 Published by Elsevier GmbH. All rights reserved.
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