期刊
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
卷 39, 期 25, 页码 13550-13556出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2014.01.145
关键词
Biohydrogen; Dark fermentation; Pretreatment method; Ionizing irradiation; Digested sludge
资金
- Program for Changjiang Scholars and Innovative Research Team in University [IRT-13026]
- Open Research Fund Program of Key Laboratory of Advanced Reactor Engineering and Safety, Ministry of Education of China
- National High Technology Research and Development Program [2009AA063905]
- National Natural Science Foundation of China [51338005]
The pretreatment of digested sludge by different methods, including ionizing irradiation, heat-shock, acid and base, was performed for enriching hydrogen-producing bacteria. These methods were evaluated and compared based on their suitability in the enrichment of hydrogen-producing bacteria in dark fermentation with glucose as a substrate in batch tests. The experimental results showed that the seed sludge pretreated by ionizing irradiation achieved the best hydrogen production among the different pretreatment methods, and the maximum hydrogen production potential, maximum hydrogen production rate, hydrogen yield and substrate degradation rate were 525.6 mL, 37.2 mL/h, 267.7 mL/g glucose (2.15 mol/mol glucose) and 98.9%, respectively. Ionizing irradiation can be a good optional pretreatment method for enriching hydrogen-producing bacteria from digested sludge. The effect of ionizing irradiation on the microbial community structure dynamics of the pretreated sludge deserves further study, which will help us to understand the mechanisms leading to the effect of high bio-hydrogen production. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
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