期刊
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
卷 39, 期 17, 页码 9012-9017出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2014.03.244
关键词
Laminaria japonica; Saccharification; Anaerobic dark fermentation; Hydrogen production
资金
- Natural Science Foundation of Tianjin, China [12JCQNJC04200]
- Foundation of Tianjin Key Laboratory of Marine Resources and Chemistry (Tianjin University of Science Technology) [201302]
In this study, one macro-alga (Laminaria japonica) was used for fermentative hydrogen production by anaerobic mixed bacteria. The saccharification efficiency and hydrogen production by L. japonica with four different pretreatment methods, including heat, acid, alkaline and ultrasonic treatment, were investigated. The results showed that the saccharification efficiency from L. japonica that was pretreated with acid was the highest among the four methods. The saccharification efficiency for the total reducing sugars in the acid-pretreated L. japonica was 350.54 +/- 19.89 mg/g (mean +/- S.E.). The cumulative hydrogen production was 66.68 +/- 5.68 mL/g from the heat-pretreated L. japonica, whereas that of L. japonica that was subjected to acid, alkaline, and ultrasonic pretreatment and the control was 43.65 +/- 6.87 mL/g, 15.00 +/- 3.89 mL/g, 23.56 +/- 4.56 mL/g and 10.00 +/- 1.21 mL/g, respectively. In addition, the effects of substrate concentration and initial pH on hydrogen production from heat-pretreated L. japonica were also analyzed. The results showed that the maximum hydrogen production was 83.45 +/- 6.96 mL/g with a hydrogen concentration of approximately 28.4% from heat-pretreated L. japonica when the initial pH and substrate concentration were determined to be 6.0 and 2%, respectively. Heat pretreatment was the most effective method for increasing fermentative hydrogen production when L. japonica was used as the only substrate. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
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