期刊
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
卷 37, 期 17, 页码 12229-12233出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2012.05.148
关键词
Hydrogen production; Reduced pigment; Transposon mutagenesis; Rhodobacter capsulatus
资金
- National Key Basic Research Program 973 Project
- MOST of China [2012CB215303, 2009CB220000]
The light shielding effect of photosynthetic bacteria in photo fermentation process, which is caused by high content of pigment, hinders their hydrogen production rates under intense light irradiation. In order to mitigate this effect and improve hydrogen production efficiency, it is necessary to screen mutants that hold less pigment content. In this study, a mini-Tn5 transposon encoded plasmid pRL27 was employed for transposon mutagenesis of Rhodobacter capsulatus SB1003. A mutant named MC1417 showed significant lower light absorbance from 330 nm to 900 nm compared to its parental strain by UV-visible spectra, and its bacteriochlorophyll a content was reduced by 38%. The results showed that its photo fermentative hydrogen production was improved by 50.5% on the basis of BChl a content using acetate and butyrate as carbon source under intense light irradiation, indicating that it is effective on improving hydrogen production by repressing the pigment biosynthesis. DNA sequencing and BLAST in NCBI Genebank showed that the mutation occurred within its pucDE gene. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
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