期刊
MICRON
卷 72, 期 -, 页码 15-20出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micron.2015.01.004
关键词
AFM; Young's modulus; Nanosecond pulsed electric fields; Jurkats; Cytoskeleton
类别
资金
- National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institute of Health [R21AR063334]
We investigated the effects of a single 60 nanosecond pulsed electric field (nsPEF) of low (15 kV/cm) and high (60 kV/cm) field strengths on cellular morphology and membrane elasticity in Jurkat cells using fluorescent microscopy and atomic force microscopy (AFM). We performed force displacement measurements on cells using AFM and calculated the Young's modulus for membrane elasticity. Differential effects were observed depending upon pulsing conditions. We found that a single nsPEF of low field strength did not induce any apparent cytoskeletal breakdown and had minor morphological changes. Interestingly, force measurements and calculation of Young's modulus showed a significant decrease in membrane elasticity. A single nsPEF of high field strength induced stark morphological changes due to disruption of the actin cytoskeleton and a marked decrease in elasticity likely caused by irreversible membrane damage. We suggest that the cellular morphology is mainly dependent on stabilization by the actin cytoskeleton, while the elasticity changes are partially dependent on the cytoskeletal integrity. (C) 2015 Elsevier Ltd. All rights reserved.
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