Bio-hydrogen production by different operational modes of dark and photo-fermentation: An overview

This article overviews reported studies on bio-hydrogen production from different raw materials by dark and photo-fermentations operated with different modes. Sequential and combined dark and photo-fermentations operated in batch, continuous and fed-batch modes were compared. Operating conditions and modes resulting in the highest hydrogen yield and formation rate were revealed. Relative advantages of sequential and combined dark and photo-fermentations were discussed. Sequential fermentation was found to be preferable due to high H-2 yields and productivities. High cell density fed-batch culture with controlled feeding and simultaneous product removal was concluded to be the most suitable operation mode at the optimum environmental conditions. Copyright (C) 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.