期刊
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
卷 34, 期 9, 页码 3647-3652出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2009.02.078
关键词
Hydrogen production; Co-culture; C. Butyricum; R. faecalis
资金
- National Natural Science Fund of China [30470054]
- National Natural Science Key Fund of China [50638020]
- National High Technology Research and Development Program of China [2006AA05Z109]
- China Postdoctoral Science Foundation [20070410910]
In this work, the effects of different parameters on co-culture hydrogen production using Clostridium Butyricum and immobilized Rhodopseudomonas faecalis RLD-53 were investigated. The maximum hydrogen yield of 4.134 mol H-2/mol glucose was obtained using 6 g/l glucose, 50 mmol/l phosphate buffer, initial pH of 7.5, the ratio of dark to photo bacteria of 1:10 and light intensity of 8000 lux. The maximum hydrogen production rate was 33.85 ml H-2/l/h. Phosphate buffer concentration was the most important parameter influencing hydrogen production in this co-culture. The ratio of acetate to butyrate increased from 0.74 to 1.82 in the soluble metabolites from C. butyricum with phosphate buffer concentration of 10-50 mmol/l. Experimental results could be of great significance for further pilot studies of co-culture hydrogen production. (C) 2009 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.
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