期刊
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
卷 33, 期 21, 页码 6092-6099出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2008.07.102
关键词
Photofermentative hydrogen production; Media optimization; Response surface methodology; Cyanobacteria; Synechocystis sp PCC 6803
资金
- Office of Science (BER)
- U.S. Department of Energy [DE-FG02-05ER64130]
- NSF
By optimizing concentrations of key nutrients in the media of Synechocystis sp. PCC 6803, we achieved nearly 150-fold greater photofermentative hydrogen (H-2) production than was achieved by analogous, sulfur-deprived cultures, which are well known to produce much more H-2 than cultures grown on complete media. This was associated with a 44-fold increase in glycogen concentration. Using response surface methodology to determine optimum conditions, we found that, instead of completely starving cells of sulfur or another essential nutrient, the highest H-2 production (0.81 +/- 0.36 mu mol H-2 mg Chl(-1) h(-1)) occurred with 0.52 mM NH4+, 20.1 mu M SO42-, and 46 mM HCO3-. H-2 profiling experiments provided initial screening of NH4+, HCO3-, SO42-, and PO43- concentrations and identified the significant variables in H-2 production to be NH4+, SO42-, and the interactions of both NH4+ and SO42- with HCO3-. Our results indicate that optimized amounts of nitrogen and sulfur in the nutrient media are superior to total deprivation of these nutrients for H-2 production. (C) 2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.
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