期刊
MICROCHEMICAL JOURNAL
卷 123, 期 -, 页码 22-27出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.microc.2015.05.013
关键词
CD-MEKC; Melatonin; Tryptophan; Serotonin; Indole-3-acetic acid; Plants
资金
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT 2189]
- University of Cuyo [6/A445]
- Consejo Nacional de Investigaciones Cientificas y Tecnologicas (CONICET) [PIP 309]
The identification of melatonin in plants has inspired new investigations to understand its biological function. In this work, a robust, reliable, low-cost, quick and simple method based on solid phase extraction followed by micellar electrokinetic chromatography for the extraction, preconcentration and simultaneous determination of melatonin, tryptophan, serotonin and indole-3-acetic add in plant material is proposed. Extraction of indole compounds from plant tissues was enhanced by ultrasound and solid phase extraction, which was carried out with C-8 SPE cartridges. The use of a dual pseudostationary phase system, involving a mixture of SDS anionic micelles and beta-cyclodextrin, enabled to reach adequate selectivity. A BCE of 10 mM sodium tetraborate (pH 9.2), containing 20 mM beta-CD, 20 mM SDS, and 10% (v/v) of acetonitrile, allowed baseline separation in less than 10 min. The proposed methodology provided limits of detection (LODs) down to low ppb levels. Under the optimal conditions, a successful application on Arabidopsis tissue, green, and linden tea leaves confirmed the validity of this method for food analysis and as a tool to contribute to the elucidation of the biological role of melatonin in plants. (C) 2015 Elsevier B.V. All rights reserved.
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