期刊
MICROBIOLOGICAL RESEARCH
卷 170, 期 -, 页码 195-204出版社
ELSEVIER GMBH
DOI: 10.1016/j.micres.2014.06.006
关键词
Enterobacter sp.; P solubilization; 2-DE; MALDI-TOF/MS; RT-PCR
类别
资金
- ICAR, New Delhi
- DST, New Delhi [P-27/115]
- J.C. Bose National Fellowship
- CSIR
- SCMAT
Proteomics and biochemical analyses were used to unravel the basis for higher growth yield of Enterobacter sp. LCR1 on insoluble phosphate medium compared to soluble. Proteomic analysis using 2-DE, MALDI-TOF/MS and LC-MS revealed the involvement of nine proteins. Down-regulation of fructose bisphosphate aldolase with decreased concentrations of glucose-6-phosphate and fructose-6-phosphate indicated diminished glycolysis. However, up-regulation of phosphoglycerate mutase, increase in the activities of 6-phosphogluconate dehydratase, 2-keto-3-deoxy-6-phosphogluconate aldolase and 6-phosphogluconate dehydrogenase suggested induction of Entner-Doudoroff and pentose phosphate pathways. These pathways generate sufficient energy from gluconic acid, which is also used for biosynthesis as indicated by up-regulation of elongation factor Tu, elongation factor G and protein disulfide isomerase. Increased reactive oxygen species (ROS) formation resulting from organic acid oxidation leads to overexpressed manganese superoxide dismutase and increased activities of catalase and ascorbate peroxidase. Thus the organism uses gluconate instead of glucose for energy, while alleviating extra ROS formation by oxidative defense enzymes. (C) 2014 Elsevier GmbH. All rights reserved.
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