期刊
MICROBIOLOGICAL RESEARCH
卷 178, 期 -, 页码 18-26出版社
ELSEVIER GMBH
DOI: 10.1016/j.micres.2015.05.008
关键词
Fibroblast; Porphyromonas gingivalis; Proteinase; Gingipain; Cytokine
类别
资金
- Swedish Heart-Lung Foundation
- Swedish Heart and Lung Association
- Foundation of Olle Engkvist
- Knowledge Foundation, Sweden
Periodontal pathogens, including Polphyromonas gingivalis, can form biofilms in dental pockets and cause inflammation, which is one of the underlying mechanisms involved in the development of periodontal disease, ultimately leading to tooth loss. Although P. gingivalis is protected in the biofilm, it can still cause damage and modulate inflammatory responses from the host, through secretion of microvesicles containing proteinases. The aim of this study was to evaluate the role of cysteine proteinases in P. gingivalis colony growth and development, and subsequent immunomodulatory effects on human gingival fibroblast. By comparing the wild type W50 with its gingipain deficient strains we show that cysteine proteinases are required by P. gingivalis to form morphologically normal colonies. The lysine-specific proteinase (Kgp), but not arginine-specific proteinases (Rgps), was associated with immunomodulation. P. gingivalis with Kgp affected the viability of gingival fibroblasts and modulated host inflammatory responses, including induction of TGF-beta 1 and suppression of CXCL8 and IL-6 accumulation. These results suggest that secreted products from P. gingivalis, including proteinases, are able to cause damage and significantly modulate the levels of inflammatory mediators, independent of a physical host-bacterial interaction. This study provides new insight of the pathogenesis of P. gingivalis and suggests gingipains as targets for diagnosis and treatment of periodontitis. (C) 2015 Elsevier GmbH. All rights reserved.
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