期刊
MICROBIOLOGICAL RESEARCH
卷 170, 期 -, 页码 1-9出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.micres.2014.09.006
关键词
Outer membrane vesicles; Bacterial secretion; Gram-negative bacteria; Host-pathogen interaction; Vaccine development
类别
资金
- DTRA [D-CZ-10-00001]
Outer membrane vesicles secreted by gram-negative bacteria play an important role in bacterial physiology as well as in virulence and host-pathogen interaction. Isolated vesicles of some bacteria have also been studied for their immunomodulatory potential in the vaccine development. However, the production of vesicles in sufficient amount, purity and reproducibility remains a critical challenge for subsequent analyses in most bacteria. In the present review methods of production, isolation, purification and quantification of outer membrane vesicles are summarized and discussed. (C) 2014 Elsevier GmbH. All rights reserved.
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