期刊
MICROBIOLOGICAL RESEARCH
卷 175, 期 -, 页码 84-92出版社
ELSEVIER GMBH
DOI: 10.1016/j.micres.2015.04.002
关键词
Listeria monocytogenes; MLST; MVLST; Virulence; Evolution
类别
资金
- National Basic Research Program (973) of China [2012CB518805]
- National Natural Science Foundation of China [31472193]
- National High Technology Research and Development Program of China [2012AA101601-6]
- Priority Academic Program Development of Jiangsu Higher Education Institutes (PAPD)
Listeria monocytogenes is one of the most important foodborne pathogens causing severe diseases with a mortality rate of 24%. However, the genetic diversity and evolution of L monocytogenes, particularly at the worldwide level, are poorly defined. In this study, we performed multilocus sequence typing (MLST) and multi virulence locus sequence typing (MVIST) for 86 L. monocytogenes strains derived from 8 countries from 1926 to 2012 in order to better understand the molecular evolution and genetic characteristics of this pathogen. A total of 13 clonal complexes (CCs) were detected, of which CC1, CC2, CD, CC7, CC9, CC4 are the most prevalent. Notably, polymorphism of housekeeping genes of isolates belong to CC1 (STs = 47) increased more rapidly over the time. MIST-based phylogenetic analysis showed that serotype 1/2b and 4b strains had an interval-type evolution pattern, while serotype 1/2a and 1/2c strains had a progressive-type evolution pattern. Furthermore, strains from temporally and geographically unrelated outbreaks in differernt countries were clustered in the same subgroup of phylogenetic tree, indicating that that L. monocytogenes developed highly similar virulence genes and genetic characteristics to adaptation in a special ecological niche. Interestingly, there was a high correlation between the population structure of MVLST and MLST among the isolates of cluster IA corresponding to CC1, CC2, CC4 and CC6 that had the highest potential to cause listeriosis outbreaks, strengthening that surveillance of these CCs is important for prevention of listeriosis. The present study offers insights into the internal relationships between the population structure, distribution and pathogenicity of L. monocytogenes. (C) 2015 Elsevier GmbH. All rights reserved.
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