期刊
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
卷 19, 期 9, 页码 1545-1549出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1111/IGC.0b013e3181a844c8
关键词
Clear cell carcinoma of the ovary; Gene expression profiling; Annexin IV
资金
- Key project of the Shanghai Health Bureau [2005ZD002]
- Chinese National Natural Science Foundation [30371482]
Objective: To investigate the genes that were differentially expressed between clear cell carcinoma (CCC) and serous carcinoma (SAC) of the ovary with complementary DNA microarray. Methods: Complementary DNA microarray was carried out in 8 CCCs and 8 SACs of the ovary. Differentially expressed genes were identified and verified by real-time polymerase chain reaction. The expression of the protein was also verified with immnunohistochemistry and Western blot in cells and tissues of ovarian CCC. Results: Comparison of the gene expression profiling identified 21 genes with more than 2-fold different expression between CCC and SAC of the ovary. The up-regulated and down-regulated genes were 9 and 12, respectively. The verification of Annexin IV in the cell line and tissues was in accordance with the result of the microarray. Conclusions: The complementary DNA microarray technique is a feasible way to explore the difference of the gene expression profiling between the 2 types of ovarian carcinoma. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker.
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