期刊
MICROBES AND INFECTION
卷 17, 期 6, 页码 462-467出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2015.03.006
关键词
Giardia lamblia; Arginase 1; Macrophages
资金
- NIH [AI 094492]
- NCATS [8 UL1 TR000101]
- [NIH-P30 CA51008]
Nitric oxide (NO) has been shown to inhibit Giardia lamblia in vitro and in vivo. This study sought to determine if Giardia infection induces arginase 1 (ARG1) expression in host macrophages to reduce NO production. Stimulations of RAW 264.7 macrophage-like cells with Giardia extract induced arginase activity. Real-time PCR and immunohistochemistry showed increased ARG1 and nitric oxide synthase 2 (NOS2) expression in mouse intestine following infection. Flow cytometry demonstrated increased numbers of macrophages positive for both ARG1 and NOS2 in lamina propria following infection, but there was no evidence of increased expression of ARG1 in these cells. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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