期刊
MICROBES AND INFECTION
卷 17, 期 5, 页码 345-352出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2015.01.003
关键词
Staphylococcus epidermidis; AI-2; Biofilm; ica; bhp
资金
- Talent Project of Anhui Agricultural University [YJ2013-6, XKTS2013-6]
- Project of Anhui Province Key Discipline of Biology [2014TSTD003]
Staphylococcus epidennidis has become the most common cause of nosocomial bacteraemia and the principal organism responsible for indwelling medical device -associated infections. Its pathogenicity is mainly due to its ability to form biofilms on the implanted medical devices. Biofilm formation is a quorum-sensing (QS)-dependent process controlled by autoinducers, which are signalling molecules. Here, we investigated the function of the autoinducer-2 (AI-2) QS system, especially the influence of AI-2 on biofilm formation in S. epidermidis RP62A. Results showed that the addition of AI-2 leads to a significant increase in biofilm formation, in contrast with previous studies which showed that AI-2 limits biofilm formation in Staphylococci. We found that AI-2 increases biofilm formation by enhancing the transcription of the ica operon, which is a known component in the AI-2-regulated biofilm pathway. In addition, we first observed that the transcript level of blip, which encodes a biofilm-associated protein, was also increased following the addition of AI-2. Furthermore, we found that, among the known biofilm regulator genes (icaR, sigB, rbsU, sarA, sarX, sarZ, clpP, agrA, abfR, arlRS, saeRS), only icaR can be regulated by AI-2, suggesting that AI-2 may regulate biofilm formation by an icaR-dependent mechanism in S. epidennidis RP62A. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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