期刊
MICROBES AND INFECTION
卷 17, 期 5, 页码 353-359出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2015.01.005
关键词
Staphylococcus aureus; Streptococcus sanguinis; Endocarditis; Intravascular defense; Bacterial conditioned medium
资金
- Excellence Initiative of the German Research Foundation [EXC 294]
- European Research Council [ERC-2011-StG 282105]
- Deutsche Forschungsgemeinschaft (DFG) [Ke700/2-1]
Antimicrobial peptides are multifunctional effector molecules of innate immunity. In this study we investigated whether endothelial cells actively contribute to innate defense mechanisms by expression of antimicrobial peptides. We therefore stimulated human umbilical vein endothelial cells (HUVEC) with inflammatory cytokines, Th17 cytokines, heat-inactivated bacteria, bacterial conditioned medium (BCM) of Staphylococcus aureus and Streptococcus sanguinis, and lipoteichoic acid (LTA). Stimulation with single cytokines induced discrete expression of human beta-defensin 3 (hBD3) by IFN-gamma or IL-1 beta and of ribonuclease 7 (RNase7) by TNF-alpha. without any effects on LL-37 gene expression. Stronger hBD3 and RNase7 induction was observed after combined stimulation with IL-1 beta, TNF-alpha and IFN-gamma and was confirmed by high hBD3 and RNase7 peptide levels in cell culture supernatants. In contrast, Th17 cytokines or stimulation with LTA did not result in AMP production. Moreover, only BCM of an invasive S. aureus bacteremia isolate induced hBD3 in HUVEC. We conclude that endothelial cells actively contribute to prevent dissemination of pathogens at the blood-tissue-barrier by production of AMPs that exhibit microbicidal and immunomodulatory functions. Further investigations should focus on tissue-specific AMP induction in different endothelial cell types, on pathogen-specific induction patterns and potentially involved pattern-recognition receptors of endothelial cells. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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