期刊
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
卷 63, 期 3, 页码 358-361出版社
INFORMA HEALTHCARE
DOI: 10.3109/09637486.2011.629177
关键词
vitexin; isovitexin; IC50
资金
- earmarked fund for Modern Agro-industry Technology Research System [nycytx-018]
A seventy percent ethanol from mung bean (Vigna radiatae L.) was extracted further with CH2Cl2, EtOAc and n-BuOH to afford four fractions: CH2Cl2-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. When using L-3,4-dihydroxyphenylalanine as the substrate for mushroom tyrosinase, the EtOAc-soluble fractions showed the highest inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guided fractionation method) from the EtOAc-soluble fractions. Vitexin and isovitexin showed high inhibitory activities, with IC50 values of 6.3 and 5.6 mg/ml, respectively. This is the first study on the active compositions of azuki beans against mushroom tyrosinase.
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