期刊
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
卷 61, 期 3, 页码 272-281出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/09637480903379478
关键词
Galactomannan; beta-mannanase; haustorium; Cocos nucifera Linn; prebiotic
资金
- Department of Biotechnology (DBT)
beta-Mannanase was extracted from coconut (Cocos nucifera Linn) haustorium and purified through ammonium sulfate precipitation and sepharose 6B-lectin affinity chromatography. Coconut beta-mannanase is an acidic protein with a pI of 3.75. The molecular mass of coconut beta-mannanase (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) was found to be 44 kDa and was confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The optimum temperature and pH for enzyme activity was 70 degrees C and 5.2. The enzyme was used for the preparation of neutraceutical dietary supplement from galactomannans of guar gum and tender coconut kernel having a beta-(1,4)-linked d-mannose backbone. Depolymerized guar gum has 92% of oligosaccharides with a degree of polymerization of 3 and 7. Tender coconut kernel has a degree of polymerization of 9-39 oligosaccharides along with disaccharides and trisaccharides. Hence this mannanase will be useful to depolymerize beta-(1,4)-linked d-mannose polysaccharides from most plant sources to produce prebiotics in a cost-effective technique.
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