Enhanced β-galactosidase production by supplementing whey with cauliflower waste

Among the five Kluyveromyces marxianus strains tested for beta-galactosidase production, K. marxianus NCIM 3465 showed maximum enzyme activity of 1.62 IU mg(-1) dry weight. Different levels (5-25%, w/v) of dried cauliflower waste were incorporated into whey to evaluate the effect of its supplementation on enzyme production. Although a marginal increase in enzyme production was seen by incorporating 5% and 10% cauliflower waste in whey, nearly 15% increase in beta-galactosidase production was observed when cauliflower waste level was increased to 20% compared with whey alone. Supplementing whey with 20% cauliflower waste also decreased the production time. Lactose concentration in whey, mainly responsible for increasing the biological oxygen demand load of the effluent water, decreased from 4.2% to nearly 0% at 24 h. Thus, this study demonstrated that both these by-products/residues could be effectively used for beta-galactosidase production at commercial scale.