期刊
METHODS
卷 77-78, 期 -, 页码 147-156出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2014.10.017
关键词
PTEN; PDZ; PTPN13; Protein tyrosine phosphatase; Protein-protein interaction
资金
- Ministerio de Educacion y Ciencia [SAF2009-10226]
- Ministerio de Economia y Competitividad (Spain and Fondo Europeo de Desarrollo Regional) [SAF2013-48812-R]
- European Union [PTPNET/MRTN-CT-2006-035830]
Protein modular interactions mediated by PDZ domains are essential for the establishment of functional protein networks controlling diverse cellular functions. The tumor suppressor PTEN possesses a C-terminal PDZ-binding motif (PDZ-BM) that is recognized by a specific set of PDZ domains from scaffolding and regulatory proteins. Here, we review the current knowledge on PTEN-PDZ domain interactions and tumor suppressor networks, describe methodology suitable to analyze these interactions, and report the binding of PTEN and the PDZ domain-containing protein tyrosine phosphatase PTPN13. Yeast two-hybrid and GST pull-down analyses showed that PTEN binds to PDZ2/PTPN13 domain in a manner that depends on the specific PTPN13 PDZ domain arrangement involving the interdomain region between PDZ1 and PDZ2. Furthermore, a specific binding profile of PTEN to PDZ2/PTPN13 domain was observed by mutational analysis of the PTEN PDZ-BM. Our results disclose a PDZ-mediated physical interaction of PTEN and PTPN13 with potential relevance in tumor suppression and cell homeostasis. (C) 2014 Elsevier Inc. All rights reserved.
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