期刊
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
卷 18, 期 3, 页码 547-557出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10942912.2013.837064
关键词
Solid-phase extraction; High performance liquid chromatography; Sugarcane molasses; Sugars; Internal standard
资金
- National Natural Science Foundation of China [51078147, 51278200]
- Guangdong Natural Science Foundation [S2012010010380]
- State Key Laboratory of Pulp and Paper Engineering (South China University of Technology) [201484]
- Guangdong Provincial Science and Technology Program [2012B091100163, 2011B090400033, 2010A010500005]
Fructose, glucose, and sucrose in sugarcane molasses are determined simultaneously by high performance liquid chromatography using maltose as internal standard with refractive index detector. The mixture of the diluted samples and internal standard was purified with Sep-Pak C-18 solid-phase extraction and filtered through a 0.22-mu m membrane before injection. The results showed that the linear ranges for fructose, glucose, and sucrose were 3.30-16.48, 1.80-9.02, and 5.94-29.70 g/L with the squared correlation coefficients (R-2) being 0.9986, 0.9987, and 0.9955, respectively. The method is simple, quantified, and time-saving for determination of sugars in sugarcane molasses.
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