期刊
METHODS
卷 75, 期 -, 页码 87-95出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2014.12.020
关键词
Autophagy; Flow cytometry; GFP-LC3; Lysosome; P62/SQSTM1
资金
- Israel Science Foundation ISF [535/11]
- German Israeli Foundation GIF [1129/157]
- ISF Legacy Heritage Fund [1309/13]
Autophagy is a dynamic catabolic process that plays a major role in sequestering and recycling cellular components in multiple physiological and pathophysiological conditions. Despite recent progress in our understanding of the autophagic process there is still a shortage of robust methods for monitoring autophagy in live cells. Flow cytometry, a reliable and unbiased method for quantitative collection of data in a high-throughput manner, was recently utilized to monitor autophagic activity in live and fixed mammalian cells. In this article we summarize the advantages and potential pitfalls of the use of flow cytometry to study autophagy. (C) 2014 Elsevier Inc. All rights reserved.
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