期刊
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
卷 16, 期 1, 页码 61-69出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10942912.2010.528111
关键词
DPPH; HPLC-DAD; Nasturtium officinale; Phenolics; TBARS
The antioxidant activities of crude extract, dichloromethane, ethyl acetate, and butanolic fractions of Nasturtium officinale were investigated using the 1,1,-diphenyl-2-picrylhydrazyl and thiobarbitaric acid reactive species methods. Total phenolics and flavonoids were determined. Rutin, chlorogenic, and caffeic acids were quantified in the crude extract by high performance liquid chromatography-photodiode array detector. The antioxidant and radical scavenging activity of the crude extract and fractions were found in the following decreasing order: butanolic fraction > ethyl acetate fraction > dichloromethane fraction > crude extract. The extent of antioxidant activities was in accordance with the amounts of phenolics and flavonoids present in the extract and fractions. Ethyl acetate and butanolic fractions caused a sharp fall in thiobarbitaric acid reactive species production, starting at the concentration of 1 mu g/mL for both fractions with IC50 6.92 +/- 0.19 and 12.07 +/- 0.45 mu g/mL, respectively. N. officinale might be a valuable antioxidant natural source and seemed to be applicable in both healthy medicine and the food industry.
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