期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 144, 期 3, 页码 367-371出版社
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2010.10.021
关键词
Arcobacter; Organic acids; Antimicrobial activity; Chicken carcass; Inhibition
资金
- Ministry of Agriculture of the Czech Republic [MZe 0002701404]
The inhibitory effects of 17 organic acids (C-2-C-16 fatty acids, sorbic, benzoic, phenylacetic, fumaric, succinic, lactic, malic and citric) on Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii were investigated by determining their IC50 values, defined as the concentration of acid at which the target DNA sequence was expressed at 50% of the positive control level in cultures incubated at 30 degrees C for 24 h. DNA was analysed by real-time PCR. The Arcobacter strains tested were inhibited by all the organic acids, with the sensitivities in the order A. skirrowii > A. cryaerophilus > A. butzleri. Eight acids with IC50 values of < 1 mg/mL against A. butzleri were tested for their effects on A. butzleri inoculated on chicken carcasses at a concentration of 5 log CFU/g of skin. Inoculated halved carcasses were immersed in solutions of the acids at 5 mg/mL for 1 min. Samples of skin were collected from carcass halves after storage at 4 degrees C for 0, 1, 2 or 3 days for enumeration of arcobacters on Muller-Hinton agar. All eight tested acids suppressed bacterial proliferation. The highest inhibitory activities were observed for benzoic, citric, malic and sorbic acids. Subsequent sensory analysis revealed benzoic acid to be the most suitable organic acid for chicken skin treatment. (C) 2010 Elsevier B.V. All rights reserved.
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