期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 141, 期 1-2, 页码 110-115出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2010.03.003
关键词
Hydrophobin; FcHyd5p; Fusarium culmorum; Gushing; Pichia pastoris; Surface active protein; Heterologous expression
资金
- German Ministry of Economics and Technology
- Arbeitsgemeinschaft industrieller Forschungsvereinigungen Otto von Guericke [AiF-FV 14551/N]
The class II hydrophobin FcHyd5p from Fusarium culmorum was heterologously expressed by Pichia pastoris. Transcription of the recombinant gene was confirmed by RT-PCR and expression of FcHyd5p was demonstrated using SDS-PAGE and immuno-staining with 6x His-tag antibodies. FcHyd5p was purified and concentrated by dialysis. isoelectric focussing and the use of ultra filtration. It was demonstrated that FcHyd5p is able to change the hydrophopic properties of surfaces rendering them wettable after coating with the supernatant of recombinant P. pastoris cultures. Furthermore, due to its surface activity, FcHyd5p was able to stabilise air bubbles in aqueous solutions. The supernatant of a culture medium containing a FcHyd5p producing P. pastoris clone remained turbid for 24 h and the foam stable for more than 72 h after the treatment with a homogeniser, whereas the liquid of the wild type strain clarified after 10 min and the foam disintegrated after 2 h. Finally it was demonstrated, that FcHyd5p can induce spontaneous overfoaming of carbonated liquids, referred to as gushing. Addition of 2 mg freeze-dried culture supernatant from a FcHyd5p producing P. pastoris clone resulted in a lost volume of 252 ml +/- 20 per 500 ml of beer (50%) and 179 ml +/- 7 per 330 ml of carbonated water (54%), respectively. Neither untreated beer/water, nor beer/water treated with freeze-dried culture supernatant from the wild type strain showed any gushing. Furthermore, addition of 215 mu g highly purified FcHyd5p resulted in a lost volume of 77 ml +/- 40 per 500 ml beer (15%). (C) 2010 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据